Effects of vitamin D and metformin on diabetic cardiomyopathy in rats with type 2 Diabetes Mellitus
Sayed DM, Amin SN, Yassa HD, Rashed LA,Tablawy N, Elattar S
Aims/hypothesis: There is evidence that suggest a protective role of vitamin D in Diabetic cardiomyopathy (DCM). Our study was designed to compare the role of vitamin D and metformin on DCM in type 2 diabetic rats.
Methods: Sixty male rats divided into five groups; control, diabetic, diabetic + metformin, diabetic + vitamin D, diabetic + metformin and vitamin D. Echocardiographic measurement of systolic and diastolic functions was performed. Blood samples analysed for serum glucose, insulin, triglycerides, cholesterol, HDL, Ca2+, Brain natriuretic peptide and procollagen III. Gene expression of vitamin D receptor (VDR), Beclin I and SERCA 2 in cardiac tissue and histopathological examination were performed.
Results: Diabetic rats developed DCM with a significant decrease in VDR, beclin I and SERCAII gene expression in cardiac tissues of diabetic rats was recorded. Treatment with metformin, vitamin D or both improved the cardiac functions, glycemic control, and lipid metabolism and increased the expression of SERCA2, Beclin 1 and VDR. Histopathological examination showed collagen fibers deposition in hearts of diabetic rats that was decreased by treatment with metformin, vitamin D or both.
Conclusion: Vitamin D or metformin administration in combination to type 2 diabetic rats resulted in synergistic action as regard most of measured parameters.