Demonstrating glycogen at various stages of developing human liver
DR. HASHMI INTKHAB C.
Ninety five percent of all the glycogen stored in the body is found in the
liver and muscles. Liver glycogen serves as a major source of blood
glucose and is of utmost importance for supply of glucose to other
organs, especially the brain. The metabolism of liver glycogen will
depend on the nutritional state of the animal/human. Forty human
fetuses (19 males and 21 females) of different gestational ages ranging
from 12th to 36th gestational weeks were procured for the research work.
Slides of liver were stained with periodic acid Schiff (PAS) stain to
demonstrate the glycogen. The Diastase PAS stain was used for the
demonstration of glycogen in combination with the PAS stain. Glycogen
granules appeared first at 15th to 16th weeks of gestation in the form of
fine granules; thereafter they increase in size till birth.